The role of neutrophils during mild and severe influenza virus infections of mice

MD Tate; LJ Ioannidis; B Croker; LE Brown; AG Brooks; PC Reading

Journal article

The role of neutrophils during mild and severe influenza virus infections of mice

MD Tate, LJ Ioannidis, B Croker, LE Brown, AG Brooks, PC Reading

Plos One | Published : 2011

DOI: 10.1371/journal.pone.0017618

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Abstract

Neutrophils have been implicated in both protective and pathological responses following influenza virus infections. We have used mAb 1A8 (anti-Ly6G) to specifically deplete LyG6high neutrophils and induce neutropenia in mice infected with virus strains known to differ in virulence. Mice were also treated with mAb RB6-8C5 (anti-Ly6C/G or anti-Gr-1), a mAb widely used to investigate the role of neutrophils in mice that has been shown to bind and deplete additional leukocyte subsets. Using mAb 1A8, we confirm the beneficial role of neutrophils in mice infected with virus strains of intermediate (HKx31; H3N2) or high (PR8; H1N1) virulence whereas treatment of mice infected with an avirulent str..

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University of Melbourne Researchers

Lorena Brown Author

Andrew Brooks Author

Patrick Reading Author

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Grants

Awarded by The National Health and Medical Research Council (NH&MRC) of Australia

Funding Acknowledgements

This study was supported by Project Grant 509230 and Program Grant 567122 from The National Health and Medical Research Council (NH&MRC) of Australia. PCR is a NH&MRC R.D. Wright Research Fellow. The Melbourne WHO Collaborating Centre for Reference and Research on Influenza is supported by the Australian Government Department of Health and Ageing. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.